Chen, JixinBremauntz, AlbertoKisley, LydiaShuang, BoLandes, Christy F.2015-07-102015-07-102013Chen, Jixin, Bremauntz, Alberto, Kisley, Lydia, et al.. "Super-Resolution mbPAINT for Optical Localization of Single-Stranded DNA." <i>ACS Applied Materials & Interfaces,</i> 5, no. 19 (2013) American Chemical Society: 9338-9343. http://dx.doi.org/10.1021/am403984k.https://hdl.handle.net/1911/80888We demonstrate the application of superlocalization microscopy to identify sequence-specific portions of single-stranded DNA (ssDNA) with sequence resolution of 50 nucleotides, corresponding to a spatial resolution of 30 nm. Super-resolution imaging was achieved using a variation of a single-molecule localization method, termed as ﾓmotion blurﾔ point accumulation for imaging in nanoscale topography (mbPAINT). The target ssDNA molecules were immobilized on the substrate. Short, dye-labeled, and complementary ssDNA molecules stochastically bound to the target ssDNA, with repeated binding events allowing super-resolution. Sequence specificity was demonstrated via the use of a control, noncomplementary probe. The results support the possibility of employing relatively inexpensive short ssDNAs to identify gene sequence specificity with improved resolution in comparison to the existing methods.engThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.Journal articlesuper-resolution microscopysingle-stranded DNAoptical mappingmbPAINTbiosensingmaterials and biointerfaceshttp://dx.doi.org/10.1021/am403984k