Wong, CynthiaPawlowski, Michal E.Forcucci, AlessandraMajors, Catherine E.Richards-Kortum, RebeccaTkaczyk, Tomasz S.2018-07-162018-07-162018Wong, Cynthia, Pawlowski, Michal E., Forcucci, Alessandra, et al.. "Development of a universal, tunable, miniature fluorescence microscope for use at the point of care." <i>Biomedical Optics Express,</i> 9, no. 3 (2018) The Optical Society: 1041-1056. https://doi.org/10.1364/BOE.9.001041.https://hdl.handle.net/1911/102431Fluorescence microscopy can be a powerful tool for cell-based diagnostic assays; however, imaging can be time consuming and labor intensive to perform. Tunable systems give the ability to electronically focus at user selected depths inside an object volume and may simplify the opto-mechanical design of the imaging system. We present a prototype of a universal, tunable, miniature fluorescence microscope built from poly(methyl methacrylate) singlets that incorporates miniature, electrowetted lenses for electronic focusing. We demonstrate the ability of this system to perform clinically relevant differential white blood cell counts using single use custom cartridges pre-loaded with the fluorescent dye acridine orange.engAn OSA-formatted open access journal article PDF may be governed by theﾠOSA Open Access Publishing Agreementﾠsigned by the author and any applicable copyright laws. Authors and readers may use, reuse, and build upon the article, or use it for text or data mining without asking prior permission from the publisher or the Author(s), as long as the purpose is non-commercial and appropriate attribution is maintained.Journal articlehttps://doi.org/10.1364/BOE.9.001041