2016-10-182016-10-182016-08-16Gonzalez, Ramon, Clomburg, James, Dellomonaco, Clementina and Miller, Elliot N., "Reverse beta oxidation pathway." Patent US9416364B2. issued 2016-08-16. Retrieved from <a href="https://hdl.handle.net/1911/91956">https://hdl.handle.net/1911/91956</a>.https://hdl.handle.net/1911/91956The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.33engUtility patentUS9416364B2