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Landes, Christy F. Salatan, Ferandre C. 2011-07-25T02:07:19Z 2011-07-25T02:07:19Z 2010 Salatan, Ferandre C.. "Single-molecule FRET analysis of the dynamics of AMPA receptor." (2010) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/62189">https://hdl.handle.net/1911/62189</a>. https://hdl.handle.net/1911/62189 THESIS CHEM. 2010 SALATAN Single-molecule fluorescence resonance energy transfer (smFRET) spectroscopy has advanced to become a powerful tool in characterizing biomolecules to the molecular level. In this thesis, the smFRET technique was utilized for the first time to analyze the dynamics of the conformational changes of the ligand-binding domain (S1S2) of GluR2, a subunit of the ionotropic glutamate/AMPA receptor. The GluR2-SIS2 molecule was determined to have a more open cleft when the natural ligand glutamate is not present as compared to when glutamate is bound to the molecule. The apo and Glutamate-bound GluR2-S1S2 molecules in solution medium were observed to have more open cleft than when they are in crystallized forms. The smFRET technique has an advantage over x-ray crystallography because it can quantitatively analyze the conformations of the molecules and the interconversions between those states. The dynamics of the GluR2-S1S2 molecule elucidates the mechanism of activation of glutamate receptors. application/pdf eng Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. Biology Neurosciences Chemistry Biochemistry Physical chemistry Single-molecule FRET analysis of the dynamics of AMPA receptor Thesis Text Chemistry Natural Sciences Rice University Masters Master of Arts open.access