2026-09-20T07:35:15Zhttps://repository.rice.edu/server/oai/request

oai:repository.rice.edu:1911/175212024-01-11T20:56:51Zcom_1911_8299col_1911_13110

Brownell, William Li, Zhiwei 2009-06-04T06:42:22Z 2009-06-04T06:42:22Z 2002 Li, Zhiwei. "Membrane tether formation from outer hair cells with optical tweezers." (2002) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/17521">https://hdl.handle.net/1911/17521</a>. https://hdl.handle.net/1911/17521 THESIS BIOENG. 2001 LI The mechanical properties of the outer hair cells (OHCs) lateral wall are essential to elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), consistent with the presence of a more extensive cytoskeleton support beneath the PM at the lateral wall. The apparent PM stiffness, which was estimated by measuring tether force at different tether length, was 3.71 pN/mum for OHC lateral wall and 4.57 pN/mum for OHC basal end. The apparent PM viscosity was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13--33 pN·s/mum. 54 p. application/pdf eng Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. Biomedical engineering Biophysics Membrane tether formation from outer hair cells with optical tweezers Thesis Text Bioengineering Engineering Rice University Masters Master of Science open.access