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McIntire, Larry V. Adams, Gretchen Louise 2009-06-04T00:40:20Z 2009-06-04T00:40:20Z 1989 Adams, Gretchen Louise. "The influence of culture environment on antigen shedding from the surface of colon cancer cells." (1989) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13336">https://hdl.handle.net/1911/13336</a>. https://hdl.handle.net/1911/13336 THESIS CH.E. 1989 ADAMS The roles of some nutrients and environmental factors on CEA synthesis and release has been determined for the LS180 colon cancer cell line. When these cells were grown on various carbohydrate sources, cells grown on mannose exhibited the highest release rates at 6.5 ng CEA/mg protein/hour. When 25mM glucose with 10% FBS was used, the release rates were 3.8 ng CEA/mg protein/hour, the lowest of any of the cultures. The effects of nutrient depletion revealed that replacing the media at 145 hours after inoculation inhibited both the rate of synthesis and release of CEA. Feeding the cells at 100 hours eliminated antigen synthesis and release. Elevating incubation temperatures from 37$\sp\circ$C for 30 minutes at 100 hours increased the rates of both CEA synthesis and release from near zero to 150 ng CEA/mg protein/hour for cells heated to 45$\sp\circ$C and 42$\sp\circ$C. (Abstract shortened with permission of author.) 60 p. application/pdf eng Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. Chemical engineering Pathology The influence of culture environment on antigen shedding from the surface of colon cancer cells Thesis Text Chemical Engineering Engineering Rice University Masters Master of Science open.access