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Olah, Glenn Allen 2007-05-09T17:59:22Z 2007-05-09T17:59:22Z 1987 Olah, Glenn Allen. "MONODOMAIN SMECTIC LIQUID CRYSTALS OF MEMBRANE LIPID WITH MODEL ION CHANNELS." (1987) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13248">https://hdl.handle.net/1911/13248</a>. https://hdl.handle.net/1911/13248 Thesis Phys. 1987 Olah Phosphatidylcholine multilayers containing $\sim$24% water by total sample weight and gramicidin/lipid molar ratios, were aligned by a mechanical stressing and low temperature annealing ($$80$\mu$ thick x 40mm$\sp2$ area) monodomain defect-free multilayers containing as many as 10$\sp{17}$ uniformly oriented gramicidin channels to be prepared. The alignment of the lipid multilayers was monitored by observing conoscopic interference patterns and orthoscopic images. The smectic defects which appeared during the alignment process were identified and dissolved. The incorporation of gramicidin in the multilayers in the form of the transmembrane channels was proven by its circular dichroism spectra (CD). The well defined CD spectrum of uniformly oriented gramicidin channels was obtained. With proof that gramicidin is in the transmembrane conducting state, it is hoped that these oriented samples will permit spectroscopic studies of the ion channel in its conducting state and diffraction studies of the channel-channel organization in the membrane. (Abstract shortened with permission of author.) application/pdf eng Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. Biophysics MONODOMAIN SMECTIC LIQUID CRYSTALS OF MEMBRANE LIPID WITH MODEL ION CHANNELS Thesis Text Physics Natural Sciences Rice University Masters Master of Arts open.access