Faculty Publications

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https://hdl.handle.net/1911/75173
This collection includes faculty journal articles deposited per Rice's Open Access Policy and additional faculty work. Items found in this collection can also be found in the authors' departmental faculty publication collections.

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Browsing Faculty Publications by Subject "3D culture"

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    Enhanced osteogenesis in co-cultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds
    (Mary Ann Liebert, Inc., 2013) Gershovich, Julia G.; Dahlin, Rebecca L.; Kasper, F. Kurtis; Mikos, Antonios G.
    In this work, human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly co-cultured with human umbilical vein endothelial cells (HUVECs) on electrospun 3D poly(-caprolactone) microfiber scaffolds in order to evaluate the co-culture’s effect on the generation of osteogenic constructs. Specifically, cells were cultured on scaffolds for up to three weeks, and the cellularity, alkaline phosphatase activity (ALP), and bone-like matrix formation were assessed. Constructs with co-cultures and monocultures had almost identical cellularity after the first week, however lower cellularity was observed in co-cultures compared to monocultures during the subsequent two weeks of culture. Scaffolds with co-cultures showed significantly higher ALP activity, glycosaminoglycan and collagen production, as well as greater calcium deposition over the course of study compared to monocultures of hMSCs. Furthermore, the osteogenic outcome was equally robust in co-cultures containing osteogenically precultured and non-precultured hMSCs. The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic culture conditions is an effective strategy to promote osteogenesis.
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    Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures
    (Wiley, 2015) Engel, Brian J.; Constantinou, Pamela E.; Sablatura, Lindsey K.; Doty, Nathaniel J.; Carson, Daniel D.; Farach-Carson, Mary C.; Harrington, Daniel Anton; Zarembinski, Thomas I.
    Validation of a high-throughput compatible 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells. The multilayered hyaluronic acid hydrogels improve drug screening predictability as evaluated with a panel of clinically relevant chemotherapeutics in both prostate and endometrial cancer cell lines compared to 2D culture.