This README.txt file was generated on 2020-08-18 by Shail Mehta

GENERAL INFORMATION

1. Title of Dataset: 

2. Author Information
	A. Principal Investigator Contact Information
		Name: Dr. K. Jane Grande-Allen
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: grande@rice.edu

	B. Associate or Co-investigator Contact Information
		Name: Shail Mehta	
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: smm24@rice.edu, shail.mehta12@protonmail.com

	C. Alternate Contact Information
		Name: Dr. Jennifer Connell
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: jp10@rice.edu

3. Date of data collection: 2018-08-01 - 2020-03-01

4. Geographic location of data collection: 6566 Main Street, Houston, TX

5. Information about funding sources that supported the collection of the data: Dunn Collaborative Award from the John S. Dunn Foundation


SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: Creative Commons License - Public Domain (CC0)

2. Links to publications that cite or use the data: N/A

3. Links to other publicly accessible locations of the data: N/A

4. Links/relationships to ancillary data sets: The files contained in this .zip file can be 3D-printed and combined with the commercial components listed in ESM_5.pdf to assemble a working prototype of a mesoscale mechanical testing device.

5. Was data derived from another source? No
	A. If yes, list source(s): 

6. Recommended citation for this dataset: N/A


DATA & FILE OVERVIEW

1. File List: 
ESM_4.zip: Compressed file containing all Computer-Aided Design part files (.sldprt, Solidworks 2020 format) and the corresponding 3D-print files (.stl) of the mesoscale mechanical testing device. The components are listed below:
Gear_Translator_Adapter (.sldprt, .stl) - Allows the 50-tooth metal gears described in the primary manuscript to attach to and interface with the Thorlabs MS1 linear translators.
MS1_Plate (.sldprt, .stl) - Forms the base chassis of the mesoscale testing device, upon which all other components are placed and attached to.
MS1_PlateSupport (.sldprt, .stl) - Attaches to the bottom of the plate in order to raise the tester body to accommodate the large gears, and also allows for the device to fit into or attach on to upright or inverted microscopy stages.
MS1_Posts_Confocal (.sldprt, .stl) - These are the force-transducing components of the device, on to which the samples are attached for testing. Lengthened to allow for mm and sub-mm scale sample attachment. Suited for microscopic sample deformation acquisition (fluorescence, confocal, Second-Harmonic Generation microscopes). Can be attached upright ore upside-down to correspond to upright or inverted microscopes.
MS1_Posts_Stereo (.sldprt, .stl) - These are the force-transducing components of the device, on to which the samples are attached for testing. Allow for mm scale sample attachment. Suited for macroscopic sample deformation acquisition (stereomicroscopes, cellphone cameras). Can be attached upright or upside-down to correspond to upright or inverted microscopes.
MS1_Reservoir (.sldprt, .stl) - Reservoir that fits into the plate to allow for hydrated testing of biological samples in Phosphate-Buffered Saline (PBS).
MS1_Reservoir_Spacers (.sldprt, .stl) - Components that form a part of the mechanism that allows the reservoir to hang freely even if not supported from the bottom.
MS1_Reservoir_Supports (.sldprt, .stl) - Components that form a part of the mechanism that allows the reservoir to hang freely even if not supported from the bottom. These parts hold up the reservoir, and threaded nuts can be slotted into them in order to allow for the user to raise or lower the reservoir to accommodate various microscopy working distances.
MS1_Translator_Alt (.sldprt, .stl) - Attaches to and translates with the Thorlabs MS1 linear translators to transfer displacement from the translator to the sample.
MS1_Upright_Spacer (.sldprt, .stl) - Acts as an extension to 'MS1_Translator_Alt' in order to lower the sample further into the reservoir when the device is configured for upright confocal microscopes. This allows for a thicker layer of fluid to sit on top of the sample for more particular microscopy methods such as Second-Harmonic Generation microscopes.
TE300_Clamp_A (.sldprt, .stl) - The first of a set of two components that attach to 'MS1_PlateSupport' in order to adapt the mesoscale testing device to microscopy stages that allow items to be screwed directly onto the stage (used for a Nikon Eclipse TE300 fluorescence microscope).
TE300_Clamp_B (.sldprt, .stl) - The second of this set of two components that attach to 'MS1_PlateSupport' in order to adapt the mesoscale testing device to microscopy stages that allow items to be screwed directly onto the stage (used for a Nikon Eclipse TE300 fluorescence microscope).

2. Relationship between files, if important: These files can be assembled together along with the commercial components listed in a separate README (ESM_5_README) to create the mesoscale testing device referenced in these README documents. 

3. Additional related data collected that was not included in the current data package: N/A

4. Are there multiple versions of the dataset? No
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
These files were generated using several versions of the Solidworks design software (2018, 2019, 2020). An initial prototype was drafted and 3D-printed, and then iteratively improved until all design criteria were sufficiently met. 

2. Methods for processing the data: N/A

3. Instrument- or software-specific information needed to interpret the data: 
Solidworks 2019 or later versions will be able to open the .sldprt files for editing, and the .stl file format is compatible with most commercial slicing programs (most notably all versions of Ultimaker Cura). If .sldprt file compatibility is an issue, the .stl files can be printed directly or opened and reverse-engineered to recreate the components.

4. Standards and calibration information, if appropriate: N/A

5. Environmental/experimental conditions: Print files were printed using an Ultimaker 2+ 3D printer. A 0.4 nozzle size was used, with a print temperature of 215 C and a bed temperature of 60 C. The Ultimaker's cardboard shipping container and aluminum foil were used as makeshift enclosure components to ensure the lowest possible warping. Force-transducing posts (Stereo and Confocal types) were printed with the cylinder oriented on its side, with supports. This ensured a clean print of the thin cylinder feature. A 24x50 mm glass coverslip was attached to the bottom of the reservoir using silicone sealant, which acted to both adhere the coverslip to the reservoir and prevent reservoir leakage.

6. Describe any quality-assurance procedures performed on the data: N/A

7. People involved with sample collection, processing, analysis and/or submission: 
Manuscript authors:
	First: Shail Maharshi Mehta
	Second: Diego Ricardo De Santos, Shweta Sridhar, Veronica Cristina Aguayo, Carlos Alberto Meraz
	Third: Mary Mikos
	Corresponding: Dr. K. Jane Grande-Allen


DATA-SPECIFIC INFORMATION FOR: ESM_4.zip

1. Number of variables: N/A

2. Number of cases/rows: N/A

3. Variable List: Independent variable: N/A

4. Missing data codes: N/A

5. Specialized formats or other abbreviations used: N/A