This README.txt file was generated on 2020-08-18 by Shail Mehta

GENERAL INFORMATION

1. Title of Dataset: 

2. Author Information
	A. Principal Investigator Contact Information
		Name: Dr. K. Jane Grande-Allen
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: grande@rice.edu

	B. Associate or Co-investigator Contact Information
		Name: Shail Mehta	
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: smm24@rice.edu, shail.mehta12@protonmail.com

	C. Alternate Contact Information
		Name: Dr. Jennifer Connell
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: jp10@rice.edu

3. Date of data collection: 2018-08-01 - 2020-03-01

4. Geographic location of data collection: 6566 Main Street, Houston, TX

5. Information about funding sources that supported the collection of the data: Dunn Collaborative Award from the John S. Dunn Foundation


SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: Creative Commons License - Public Domain (CC0)

2. Links to publications that cite or use the data: N/A

3. Links to other publicly accessible locations of the data: N/A

4. Links/relationships to ancillary data sets: The files contained in this .zip file can be purchased and combined with the 3D-printed components contained in ESM_4.zip to assemble a working prototype of a mesoscale mechanical testing device.

5. Was data derived from another source? No
	A. If yes, list source(s): 

6. Recommended citation for this dataset: N/A


DATA & FILE OVERVIEW

1. File List: 
ESM_5.pdf: PDF document containing all commercial components of the mesoscale mechanical testing device.

2. Relationship between files, if important: These files can be assembled together along with the 3D-printed components listed in a separate README (ESM_4_README) to create the mesoscale testing device referenced in these README documents. 

3. Additional related data collected that was not included in the current data package: N/A

4. Are there multiple versions of the dataset? No
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
These components were used in tandem with the 3D printed components described in ESM_4.zip. An initial prototype was drafted and 3D-printed, and then iteratively improved until all design criteria were sufficiently met. This iteration process resulted in the final parts list described in this document.

2. Methods for processing the data: N/A

3. Instrument- or software-specific information needed to interpret the data: 
Details regarding notable components and assembly instructions are listed below:
Thorlabs MS1 Linear Translator - The translator is meant to be attached to 'MS1_Plate' upside-down. This is because the translator restricts pulling forces in one direction, but not the other. Attaching the translator upside-down ensures that forces from a sample undergoing testing do not pull the translator arm out of position.
NEMA 11 Stepper Motor - There are attachment sites on 'MS1_Plate' that conform almost exactly to the shape of these motors. The motors are held in place using short 2-56 screws.
KHK SSY1-12A - This metric gear is meant to fit the 5mm shaft of the NEMA 11 stepper motor exactly. It comes with a set screw, resulting in a snug and aligned fit.
KHK SSY1-50A - This metric gear is meant to fit onto the MS1 linear translator via the 3D printed component 'Gear_Translator_Adapter'. A good balance of tightening is required using the included set screw - too loose and the translator shaft will not spin with the gear; too tight and the plastic adapter will deform, resulting in slightly off-axis rotation.
Romeo Mini BLE Controller - Online packages exist to deliver commands to the controller via Bluetooth. These packages were modified to send commands to the NEMA 11 stepper motors germane to mechanical testing.
0.086" Diameter 2.125" Long Steel Rod - These rods are sent through the 'MS1_Posts_Confocal' or 'MS1_Posts_Stereo' print files, which contain clear holes for them. Typically, the clear hole printed directly onto the build plate will be slightly smaller than the clear hole on the other side. Therefore, care should be taken to manually expand the smaller clear hole by sending the rods through it several times before attachment. These rods work to ensure alignment between the posts, which is crucial for microscopy.
1" Long 0.25" Diameter Extension Spring - These springs hook on to the 'MS1_Posts_Confocal' or 'MS1_Posts_Stereo' print files, which contain hook points for them. The extension springs work as a preload to ensure smoother translation.

4. Standards and calibration information, if appropriate: N/A

5. Environmental/experimental conditions: N/A

6. Describe any quality-assurance procedures performed on the data: N/A

7. People involved with sample collection, processing, analysis and/or submission: 
Manuscript authors:
	First: Shail Maharshi Mehta
	Second: Diego Ricardo De Santos, Shweta Sridhar, Veronica Cristina Aguayo, Carlos Alberto Meraz
	Third: Mary Mikos
	Corresponding: Dr. K. Jane Grande-Allen


DATA-SPECIFIC INFORMATION FOR: ESM_5.pdf

1. Number of variables: N/A

2. Number of cases/rows: N/A

3. Variable List: Independent variable: N/A

4. Missing data codes: N/A

5. Specialized formats or other abbreviations used: N/A