This README.txt file was generated on 2020-08-18 by Shail Mehta

GENERAL INFORMATION

1. Title of Dataset: 

2. Author Information
	A. Principal Investigator Contact Information
		Name: Dr. K. Jane Grande-Allen
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: grande@rice.edu

	B. Associate or Co-investigator Contact Information
		Name: Shail Mehta	
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: smm24@rice.edu, shail.mehta12@protonmail.com

	C. Alternate Contact Information
		Name: Dr. Jennifer Connell
		Institution: Rice University
		Address: 6566 Main Street, Houston, TX, 77030
		Email: jp10@rice.edu

3. Date of data collection: 2018-08-01 - 2020-03-01

4. Geographic location of data collection: 6566 Main Street, Houston, TX

5. Information about funding sources that supported the collection of the data: Dunn Collaborative Award from the John S. Dunn Foundation


SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: Creative Commons License - Public Domain (CC0)

2. Links to publications that cite or use the data: N/A

3. Links to other publicly accessible locations of the data: N/A

4. Links/relationships to ancillary data sets: N/A

5. Was data derived from another source? No
	A. If yes, list source(s): 

6. Recommended citation for this dataset: N/A


DATA & FILE OVERVIEW

1. File List: 
ESM_2_A.mp4: Video file demonstrating how MATLAB program identifies relevant pixels to track from video of material testing performed on custom mesoscale testing device. Acquired using Fluorescence microscope at 10x magnification. Sample is 1:10 Polydimethylsiloxane (PDMS).
ESM_2_B.mp4: Video file demonstrating how MATLAB program tracks post deflection from video of material testing performed on custom mesoscale testing device in order to determine material properties. Acquired using Fluorescence microscope at 10x magnification. Sample is 1:10 Polydimethylsiloxane (PDMS).

2. Relationship between files, if important: The pixels identified in ESM_2_A.mp4 were used to perform the post deflection tracking in ESM_2_B.mp4.

3. Additional related data collected that was not included in the current data package: N/A

4. Are there multiple versions of the dataset? No
	A. If yes, name of file(s) that was updated: 
		i. Why was the file updated? 
		ii. When was the file updated? 


METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
Top-down video of a sample undergoing strain on a custom mechanical testing device was processed using custom MATLAB code. The video was broken down into its component frames and the pixels of each frame were analyzed. Pixels with colors belonging to the point of interest were identified and displayed in ESM_2_A.mp4. Pixels corresponding to the edges of the tracked motion were averaged, and this edge point was tracked across all frames for all points of interest. The displacement calculated via tracking and the expected displacement purely from motor motion was quantified to determine post deflection. Edge tracking is demonstrated in ESM_2_B.mp4. 

2. Methods for processing the data: 
Data collected from ESM_2_B.mp4 takes the form of post deflection on either side of the sample in mm, and the displacement of the sample under strain. Post deflection undergoes conversion using an experimental calibration in order to obtain force. This measurement of force from the sample was converted to stress using sample geometry, and the displacement of the sample was also converted to strain. This generated a stress-strain curve and a measurement of sample stiffness via elastic modulus.

3. Instrument- or software-specific information needed to interpret the data: 
MATLAB R2017b was used to execute the custom code. No external packages are required.

4. Standards and calibration information, if appropriate: Plastic 3D printed posts were used as force transducers. An experimental calibration was used to convert deflection in mm to force in N. One calibration methodology was to use Polydimethylsiloxane (PDMS) test strips of known material property to correlate deflection and force. The other calibration methodology was to attach pairs of these posts directly to a commercial mechanical testing device, with a strip of paper connecting the post ends where the sample would be. Forces measured by the load cell upon pulling the two posts apart were correlated to the measured translation, which was equivalent to post deflection. As the first calibration method was more time-consuming but more accurate, the second calibration approach was used after scaling the calibration value thus obtained using the first approach.

5. Environmental/experimental conditions: Room temperature dry conditions used for testing synthetic samples. Room temperature hydrated conditions used for testing biological samples.

6. Describe any quality-assurance procedures performed on the data: New post pairs were used for each sample. Calibration was performed on each post pair before testing. Mechanical testing on data obtained using mesoscale testing device was compared with data obtained using commercial mechanical testing device for several different sample types. 

7. People involved with sample collection, processing, analysis and/or submission: 
Manuscript authors:
	First: Shail Maharshi Mehta
	Second: Diego Ricardo De Santos, Shweta Sridhar, Veronica Cristina Aguayo, Carlos Alberto Meraz
	Third: Mary Mikos
	Corresponding: Dr. K. Jane Grande-Allen


DATA-SPECIFIC INFORMATION FOR: ESM_2_A.mp4, ESM_2_B.mp4

1. Number of variables: N/A

2. Number of cases/rows: N/A

3. Variable List: N/A

4. Missing data codes: N/A

5. Specialized formats or other abbreviations used: N/A